PACE™ (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of Single Nucleotide Polymorphisms (SNPs) and insertion / deletions (indels).

The PACE™ genotyping chemistry is comprised of two parts:

1. SNP-specific assay mix; comprising two allele-specific forward primers and one common, reverse primer.

2. Universal PACE™ Genotyping Master Mix; containing all components required for PCR and generation of fluorescent signals.

When combined and mixed with template DNA, these components create a PACE™ genotyping reaction.

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Genotyping Master Mix and more...

PACE™ all versions

PACE™

PACE™ Master Mix is a universal master mix designed for use with allele-specific assays

User manual

PACE-IR™

PACE-IR™ Master Mix is a version of PACE™ Genotyping Master Mix, formulated to neutralise the effect of PCR inhibitors in crude DNA extractions

User manual

PACE™ 2.0

PACE™ 2.0 Master Mix is a version of PACE™, formulated to neutralise the effect of PCR inhibitors in crude DNA extractions, increasing performance with increase signal to noise ratio and enables users to follow reactions in real time.

User manual

PACE™ MULTIPLEX

PACE™ Multiplex Master Mix is an enhanced version of PACE™ Master Mix, formulated to enable users to analyse up to four targets in one reaction well :

2 SNPs, or 1 reference gene and a further three events of interest

User manual

PACE-RT™

PACE-RT™ Master Mix is a universal master mix designed for use with allele-specific assays, including Reverse Transcriptase step

User manual

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